Project

Part:BBa_K1230015:Design

Designed by: Andrés Ferriño Iriarte   Group: iGEM13_Ciencias-UNAM   (2013-09-26)


araC-pBad-rbs-LL37-rbs-GFP-pLacI-rbs-MarA Generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1164
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 999
    Illegal AgeI site found at 2629
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2028
    Illegal SapI site found at 981


Design Notes

LL-37 has the codon usage for humans. Using E. coli codon usage may inhibit protein function by wrong folding.


Source

Genscript 2013 DNA distribution

References